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Anti-E. coli groES Polyclonal Antibody (JN912014)

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概述
货号JN912014
品牌abinScience
描述
Anti-E. coli groES Polyclonal Antibody (JN912014) is a rabbit polyclonal antibody detecting groES. Suitable for strain K12.
种属反应性Escherichia coli
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant E. coli groES (Met1-Ala97).
靶标10 kDa chaperonin, Chaperonin-10, Co-chaperonin GroES, Cpn10, groES, groS, mopB
内毒素水平Please contact with the lab for this information.
纯化方式Purified by antigen affinity column.
Accession号P0A6F9
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

重悬Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Co-chaperonin GroES is a ~10 kDa protein. Together with the chaperonin GroEL, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding, probably by preventing aggregation and by entropically destabilizing folding intermediates. GroES binds to the apical surface of the GroEL ring, thereby capping the opening of the GroEL channel. Regulates the ATPase activity of GroEL.

1. Goloubinoff, P. et al. (1989) Nature 342, 884-9. PMID: 10532860
2. Tang, YC. et al. (2006) Cell 125, 903-14. PMID: 16751100
3. Martin, J. et al. (1991) Nature 352, 36-42. PMID: 1676490
4. Tang, YC. et al. (2008) The EMBO journal 27, 1458-68. PMID: 18418386
5. Apetri, AC. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 17351-5. PMID: 18987317
6. Chakraborty, K. et al. (2010) Cell 142, 112-22. PMID: 20603018
7. Gupta, AJ. et al. (2014) Journal of molecular biology 426, 2739-54. PMID: 24816391
8. Kusukawa, N. et al. (1989) The EMBO journal 8, 3517-21. PMID: 2573517
9. Hemmingsen, SM. et al. (1988) Nature 333, 330-4. PMID: 2897629
NoteFor research use only
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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