Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
产品使用信息
应用方法
稀释比例
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
CD5 antigen-like (CD5L) is a ~38 kDa protein. Secreted protein that acts as a key regulator of lipid synthesis: mainly expressed by macrophages in lymphoid and inflamed tissues and regulates mechanisms in inflammatory responses, such as infection or atherosclerosis. Able to inhibit lipid droplet size in adipocytes. Following incorporation into mature adipocytes via CD36-mediated endocytosis, associates with cytosolic FASN, inhibiting fatty acid synthase activity and leading to lipolysis, the degradation of triacylglycerols into glycerol and free fatty acids (FFA). CD5L-induced lipolysis occurs with progression of obesity: participates in obesity-associated inflammation following recruitment of inflammatory macrophages into adipose tissues, a cause of insulin resistance and obesity-related metabolic disease. Regulation of intracellular lipids mediated by CD5L has a direct effect on transcription regulation mediated by nuclear receptors ROR-gamma (RORC).
1. Sanjurjo, L. et al. (2015) Journal of leukocyte biology 98, 173-84. PMID: 26048980
2. Kurokawa, J. et al. (2010) Cell metabolism 11, 479-92. PMID: 20519120
3. Iwamura, Y. et al. (2012) Biochemical and biophysical research communications 422, 476-81. PMID: 22579686
4. Kurokawa, J. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 12072-7. PMID: 21730133
5. Wang, C. et al. (2015) Cell 163, 1413-27. PMID: 26607793
6. Gaublomme, JT. et al. (2015) Cell 163, 1400-12. PMID: 26607794
10. Arai, S. et al. (2016) Nature medicine 22, 183-93. PMID: 26726878
