Anti-Human UBQLN4 Polyclonal Antibody (HP715014)

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50ug
100ug

数量：

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概述
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参考文献
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概述

货号

HP715014

品牌

abinScience

描述

Anti-Human UBQLN4 Polyclonal Antibody (HP715014) is a rabbit polyclonal antibody detecting Ubiquilin-4; Ataxin-1 interacting ubiquitin-like protein; A1Up; Ataxin-1 ubiquitin-like-interacting protein A1U; Connexin43-interacting protein of 75 kDa; CIP75; UBQLN4; C1orf6; CIP75; UBIN. Suitable for Mouse and Human.

Highlights

●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human UBQLN4 (Pro12-Lys83).

靶标

Ubiquilin-4; Ataxin-1 interacting ubiquitin-like protein; A1Up; Ataxin-1 ubiquitin-like-interacting protein A1U; Connexin43-interacting protein of 75 kDa; CIP75; UBQLN4; C1orf6; CIP75; UBIN

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

Q9NRR5

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

背景

Ubiquilin-4 (UBQLN4) is a ~63 kDa protein. Regulator of protein degradation that mediates the proteasomal targeting of misfolded, mislocalized or accumulated proteins. Acts by binding polyubiquitin chains of target proteins via its UBA domain and by interacting with subunits of the proteasome via its ubiquitin-like domain. Key regulator of DNA repair that represses homologous recombination repair: in response to DNA damage, recruited to sites of DNA damage following phosphorylation by ATM and acts by binding and removing ubiquitinated MRE11 from damaged chromatin, leading to MRE11 degradation by the proteasome. MRE11 degradation prevents homologous recombination repair, redirecting double-strand break repair toward non-homologous end joining (NHEJ). Specifically recognizes and binds mislocalized transmembrane-containing proteins and targets them to proteasomal degradation.

1. Riley, BE. et al. (2004) The Journal of biological chemistry 279, 42290-301. PMID: 15280365

2. Suzuki, R. et al. (2016) EMBO reports 17, 842-57. PMID: 27113755

3. Hirayama, S. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E4199-E4208. PMID: 29666234

4. Jachimowicz, RD. et al. (2019) Cell 176, 505-519.e22. PMID: 30612738

5. Lee, DY. et al. (2013) EMBO reports 14, 373-81. PMID: 23459205

Note

For research use only

图片

Western blot

Lane 1: Hela cell lysate
Lane 2: 293T cell lysate
Lane 3: MCF-7 cell lysate
Lane 4: K562 cell lysate

参考文献

产品说明书

Anti-Human UBQLN4 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human UBQLN4 Polyclonal Antibody (HP715014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human UBQLN4 Polyclonal Antibody (HP715014)

概述

图片

参考文献

产品说明书

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