Anti-DRP1/DNM1L Polyclonal Antibody (HT429014) is a rabbit polyclonal antibody detecting DNM1L in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Danio rerio.
Highlights
●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.
种属反应性
Human, Mouse, Rat
应用
ELISA, IHC, WB
宿主
Rabbit
同种型
IgG
克隆类型
Polyclonal
免疫原
E. coli - derived recombinant Human DRP1/DNM1L (Met1-Arg516).
靶标
Dynamin family member proline-rich carboxyl-terminal domain less, DVLP, Dynamin-like protein IV, Dynamin-related protein 1, Dynamin-like protein, DLP1, Dymple, Dynamin-1-like protein, HdynIV, Dnm1p/Vps1p-like protein, DRP1, DNM1L, Dynamin-like protein 4
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
Dynamin-1-like protein (DRP1/DNM1L) is a ~81 kDa protein. Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into membrane-associated tubular structures that wrap around the scission site to constrict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. The specific recruitment at scission sites is mediated by membrane receptors like MFF, MIEF1 and MIEF2 for mitochondrial membranes. While the recruitment by the membrane receptors is GTP-dependent, the following hydrolysis of GTP induces the dissociation from the receptors and allows DNM1L filaments to curl into closed rings that are probably sufficient to sever a double membrane. Acts downstream of PINK1 to promote mitochondrial fission in a PRKN-dependent manner.
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