Anti-CD3 epsilon Recombinant Antibody (SRR2880) (HY057073)

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50ul
100ul

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货号	HY057073
品牌	abinScience
种属反应性	Human
应用	IF, FCM, WB
宿主	Rabbit
克隆类型	Monoclonal
同种型	IgG
克隆号	SRR2880
经测试应用	IF, FCM, WB
靶标	CD3E, CD3e, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E
内毒素水平	Please contact with the lab for this information.
纯度	>95% as determined by SDS-PAGE.
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	P07766
状态	Liquid
保存溶液	0.01M PBS pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note	For research use only

Western blot

Western blot analysis was performed using anti-CD3 epsilon monoclonal antibody at 1 ug/mL on various samples.
Lane 1: Jurkat cell lysate
Immunofluorescence

Jurkat cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting CD3 epsilon (HY057073) at a concentration of 20 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) at a concentration of 6.5 ug/ml for 50 minutes at room temperature. The localization of CD3 epsilon was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Flow cytometry

Flow-cytometry using anti-Human CD3 epsilon antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-Human CD3 epsilon monoclonal antibody (Catalog HY057073, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Rabbit IgG H&L Polyclonal Antibody, FITC (abinScience:TF690414) and cells analysed on a NovoCyte Flow Cytometer.

产品说明书

Anti-CD3 epsilon Recombinant Antibody (SRR2880).pdf

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Anti-CD3 epsilon Recombinant Antibody (SRR2880) (HY057073)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-CD3 epsilon Recombinant Antibody (SRR2880) (HY057073)

概述

图片

参考文献

产品说明书

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