| 背景 | This is bulk anti-human HLA-DP antibody, clone B7/21, a mouse monoclonal of the IgG3 isotype raised against human HLA-DP. The clone is a long-established, well-characterised reagent widely used to identify and study the HLA-DP subset of human MHC class II molecules, and it is validated here for Western blot, immunocytochemistry, and flow cytometry. Supplied in bulk milligram-to-gram quantities and manufactured to a low-endotoxin, research-grade standard, it is well suited to high-throughput immunophenotyping, cell sorting and enrichment workflows, tissue and cell-line screening, and biochemical characterisation of class II expression. Its human-restricted reactivity and IgG3 isotype make it appropriate for flow-based detection of antigen-presenting cells and for functional in-vitro studies of DP-restricted antigen presentation and T-cell responses. As a bulk RUO product it offers lot consistency for scaled experiments and assay development. For research use only; not for diagnostic or therapeutic use.
HLA-DP is one of the three classical human MHC class II isotypes, alongside HLA-DR and HLA-DQ. It is a heterodimer of a membrane-anchored alpha chain (encoded by HLA-DPA1) and a highly polymorphic beta chain (HLA-DPB1), which together form a peptide-binding groove that presents exogenous, endosomally processed peptides to CD4+ T cells. HLA-DP is expressed constitutively on professional antigen-presenting cells including dendritic cells, B lymphocytes, macrophages and thymic epithelium, and can be induced on other cell types by interferon-gamma. Historically considered less abundant and less studied than HLA-DR, HLA-DP is now recognised for its role in transplantation matching, allorecognition and graft-versus-host disease, in susceptibility to autoimmune and infectious diseases, and as a source of tumour-associated neoantigen presentation. The UniProt reference for the alpha chain is P20036 (HLA-DPA1). The HLA-DP antigen is present on approximately 10% of normal peripheral blood lymphocytes but not on resting peripheral T-lymphocytes. HLA-DP is present in low density on peripheral blood monocytes and mitogen-stimulated T-lymphoblasts. Anti-HLA-DP reacts with most B-cell lines. |
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