| 背景 | This is a mouse monoclonal antibody (clone 1a3, mouse IgG2a) raised against human HLA-DQ, supplied in bulk (mg to gram scale) for research use only. As an anti-human MHC class II reagent validated for Western blot and ELISA, it suits biochemical and immunochemical characterization of HLA-DQ expression rather than in-vivo functional studies. The IgG2a format offers robust binding for denatured or native antigen detection depending on the assay. Bulk availability supports large or repeated experiments, assay development, and screening workflows where reagent-lot consistency matters. Like other ichor.bio research antibodies, it is offered low-endotoxin and RUO-grade, which is advantageous for cell-based and functional assays where endotoxin contamination could confound readouts, even though this particular clone's primary applications are biochemical. Because the target is human and the listed applications are WB and ELISA, this reagent is positioned for expression analysis, transplantation immunology, autoimmunity, and HLA typing-adjacent research rather than mouse in-vivo depletion or blockade experiments. Investigators should validate performance in their own system and antigen context.
HLA-DQ is a heterodimeric MHC class II molecule composed of an alpha chain (HLA-DQA1) and a beta chain (HLA-DQB1), expressed constitutively on professional antigen-presenting cells including dendritic cells, B lymphocytes, macrophages, and thymic epithelium, and inducibly on other cell types by interferon-gamma. It presents exogenously derived and endosomally processed peptides to CD4+ helper T cells, shaping adaptive immune responses. HLA-DQ is among the most polymorphic human loci, and specific allelic combinations are strongly associated with autoimmune diseases such as type 1 diabetes and celiac disease, as well as with transplant matching and rejection. The UniProt entry P01906 corresponds to the DQ alpha-1 chain. Because of its central role in peptide presentation and its extensive polymorphism, HLA-DQ is a recurring focus in transplantation immunology, autoimmunity, and immunogenetics research. The HLA-DQ antigen is present on approximately 10% of peripheral blood lymphocytes. This antibody reacts with virtually all B-cell lines and some tissue macrophages and dendritic cells. |
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