Anti-Mouse IFN Alpha (TIF-3C5) In Vivo Antibody - Low Endotoxin (ICH1135-100mg)

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

This antibody is the Armenian hamster monoclonal clone TIF-3C5 (Armenian Hamster IgG1) raised against mouse interferon alpha (IFN alpha). As a hamster anti-mouse reagent it is well suited to studies in mouse models, where it recognises and neutralises secreted mouse IFN alpha subtypes rather than a cell-surface antigen, making it useful for functional blocking experiments and for detecting IFN alpha by Western blot. The clone is validated for Western Blot and blocking applications. Supplied in a low-endotoxin, in-vivo-ready format (research grade <1 EU/mg; ultra-low <0.5 EU/mg) and available in bulk milligram to gram quantities, it is intended for functional and in-vivo work where minimising endotoxin-driven confounding of innate immune readouts is important. The Armenian hamster IgG1 framework allows use alongside polyclonal Armenian hamster IgG isotype controls. All material is for research use only (RUO). Researchers typically identify this reagent by the clone designation TIF-3C5. Interferon alpha (IFN alpha) is a family of type I interferons produced predominantly by plasmacytoid dendritic cells and other cells in response to viral nucleic acids and other innate immune triggers. In mice the IFN alpha family comprises numerous subtypes that signal through the shared heterodimeric type I interferon receptor (IFNAR1/IFNAR2), activating the JAK-STAT pathway and driving expression of interferon-stimulated genes. This programme establishes an antiviral state, modulates antigen presentation, and shapes both innate and adaptive immune responses. Type I interferon signalling contributes to antitumour immunity and to the pathology of several autoimmune and inflammatory conditions, including lupus-like disease, where chronic IFN alpha production sustains an interferon signature. Neutralising IFN alpha directly (as opposed to blocking the receptor) allows investigators to probe the specific contribution of the alpha subtypes within the broader type I interferon system.