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Anti-TBX3 Polyclonal Antibody (HT202014)

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  • 50ug
  • 100ug
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  • 参考文献

  • 产品说明书

概述
货号HT202014
品牌abinScience
描述
Anti-TBX3 Polyclonal Antibody (HT202014) is a rabbit polyclonal antibody detecting TBX3 in ELISA, IHC, WB. Suitable for Mouse, Dog, Danio rerio, Chicken, and Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
种属反应性Human, Mouse, Rat
应用ELISA, IHC, WB
宿主Rabbit
同种型IgG
克隆类型Polyclonal
免疫原E. coli - derived recombinant Human TBX3 (Leu107-Thr220).
靶标TBX3, T-box transcription factor TBX3, T-box protein 3
内毒素水平Please contact with the lab for this information.
纯化方式Purified by antigen affinity column.
Accession号O15119
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

T-box transcription factor TBX3 is a ~79 kDa protein. Transcriptional repressor involved in developmental processes. Binds to the palindromic T site 5'-TTCACACCTAGGTGTGAA-3' DNA sequence, or a half-site, which are present in the regulatory region of several genes. Probably plays a role in limb pattern formation. Required for mammary placode induction, and maintenance of the mammary buds during development. Involved in branching morphogenesis in both developing lungs and adult mammary glands, via negative modulation of target genes; acting redundantly with TBX2.

1. He, Ml. et al. (1999) Proceedings of the National Academy of Sciences of the United States of America 96, 10212-7. PMID: 10468588
2. Lingbeek, ME. et al. (2002) The Journal of biological chemistry 277, 26120-7. PMID: 12000749
3. Martin, N. et al. (2012) The EMBO journal 31, 95-109. PMID: 22002537
NoteFor research use only.
图片
  • Western blot

    Western blot analysis was performed using anti-IBX3 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse lung lysate
    Lane 2: Rat lung lysate

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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