Dihydrofolate reductase (DHFR) is a ~21 kDa protein. Catalyzes the reduction of 7,8-dihydrofolate (DHF) to 5,6,7,8-tetrahydrofolate in a NADPH-dependent manner. Key enzyme in folate metabolism. Contributes to the nuclear and mitochondrial de novo thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2. 1. Klon, AE. et al. (2002) Journal of molecular biology 320, 677-93. PMID: 120969172. Cody, V. et al. (2004) Acta crystallographica. Section D, Biological crystallography 60, 646-55. PMID: 150395523. Reynolds, RC. et al. (2007) Journal of medicinal chemistry 50, 3283-9. PMID: 175695175. Volpato, JP. et al. (2009) The Journal of biological chemistry 284, 20079-89. PMID: 194780826. McEntee, G. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 15157-62. PMID: 218761847. Gangjee, A. et al. (1998) Journal of medicinal chemistry 41, 3426-34. PMID: 97195958. Anderson, DD. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 15163-8. PMID: 218761889. Anderson, DD. et al. (2012) The Journal of biological chemistry 287, 7051-62. PMID: 22235121 | 
