Anti-PGK1 Polyclonal Antibody (HF674014)

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概述
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产品说明书

概述

货号

HF674014

品牌

abinScience

描述

Anti-PGK1 Polyclonal Antibody (HF674014) is a rabbit polyclonal antibody detecting PGK1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Cricetulus griseus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human PGK1 (Ser2-Ile417).

靶标

PRP 2, Primer recognition protein 2, PGKA, PGK1, Phosphoglycerate kinase 1, Cell migration-inducing gene 10 protein

纯化方式

Purified by antigen affinity column.

Accession号

P00558

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Phosphoglycerate kinase 1 (PGK1) is a ~44 kDa protein. Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. Both L- and D- forms of purine and pyrimidine nucleotides can be used as substrates, but the activity is much lower on pyrimidines. In addition to its role as a glycolytic enzyme, it seems that PGK1 acts as a polymerase alpha cofactor protein (primer recognition protein). Acts as a protein kinase when localized to the mitochondrion where it phosphorylates pyruvate dehydrogenase kinase PDK1 to inhibit pyruvate dehydrogenase complex activity and suppress the formation of acetyl-coenzyme A from pyruvate, and consequently inhibit oxidative phosphorylation and promote glycolysis. May play a role in sperm motility.

1. Bollong, MJ. et al. (2018) Nature 562, 600-604. PMID: 30323285

2. Fujii, H. et al. (1980) The Journal of biological chemistry 255, 6421-3. PMID: 7391028

3. Gondeau, C. et al. (2008) Nucleic acids research 36, 3620-9. PMID: 18463139

4. Jindal, HK. et al. (1990) The Journal of biological chemistry 265, 6540-3. PMID: 2324090

5. Li, X. et al. (2016) Molecular cell 61, 705-719. PMID: 26942675

6. Chen, Z. et al. (2023) Nature communications 14, 1121. PMID: 36849569

7. Liu, XX. et al. (2016) Human reproduction (Oxford, England) 31, 273-9. PMID: 26677959

Note

For research use only.

图片

参考文献

产品说明书

Anti-PGK1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

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Anti-PGK1 Polyclonal Antibody (HF674014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-PGK1 Polyclonal Antibody (HF674014)

概述

图片

参考文献

产品说明书

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