Anti-MB Polyclonal Antibody (HF785014)

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概述
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参考文献
产品说明书

概述

货号

HF785014

品牌

abinScience

描述

Anti-MB Polyclonal Antibody (HF785014) is a rabbit polyclonal antibody detecting MB in ELISA, IHC, WB. Suitable for Human, Aotus trivirgatus, Balaena mysticetus, Balaenoptera edeni, and Callithrix jacchus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human MB (Met1-Gly154).

靶标

Myoglobin, MB

纯化方式

Purified by antigen affinity column.

Accession号

P02144

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Myoglobin (MB) is a ~17 kDa protein. Monomeric heme protein which primary function is to store oxygen and facilitate its diffusion within muscle tissues. Reversibly binds oxygen through a pentacoordinated heme iron and enables its timely and efficient release as needed during periods of heightened demand. Depending on the oxidative conditions of tissues and cells, and in addition to its ability to bind oxygen, it also has a nitrite reductase activity whereby it regulates the production of bioactive nitric oxide. Under stress conditions, like hypoxia and anoxia, it also protects cells against reactive oxygen species thanks to its pseudoperoxidase activity.

1. Olivé, M. et al. (2019) Nature communications 10, 1396. PMID: 30918256

2. Hofbauer, S. et al. (2022) The FEBS journal 289, 1105-1117. PMID: 34679218

3. Quesnelle, K. et al. (2020) Nitric oxide : biology and chemistry 104-105, 36-43. PMID: 32891753

Note

For research use only.

图片

参考文献

产品说明书

Anti-MB Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-MB Polyclonal Antibody (HF785014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-MB Polyclonal Antibody (HF785014)

概述

图片

参考文献

产品说明书

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