Anti-EDN1/Endothelin1 Polyclonal Antibody (HY182014)

价格:

规格：

50ug
100ug

数量：

联系我们

一键复制产品信息

概述
图片
参考文献
产品说明书

概述

货号

HY182014

品牌

abinScience

描述

Anti-EDN1/Endothelin1 Polyclonal Antibody (HY182014) is a rabbit polyclonal antibody detecting Endothelin1 in ELISA, IHC, WB. Suitable for Human and Macaca fascicularis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human EDN1 (Ala18-Trp212).

靶标

Endothelin-1, EDN1, PPET1, ET-1, Preproendothelin-1, Endothelin1

纯化方式

Purified by antigen affinity column.

Accession号

P05305

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Endothelin-1 (EDN1) is a ~24 kDa protein. Endothelins are endothelium-derived vasoconstrictor peptides. Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells. Also binds the DEAR/FBXW7-AS1 receptor. Promotes mesenteric arterial wall remodeling via activation of ROCK signaling and subsequent colocalization of NFATC3 with F-actin filaments. NFATC3 then translocates to the nucleus where it subsequently promotes the transcription of the smooth muscle hypertrophy and differentiation marker ACTA2.

1. Rufanova, VA. et al. (2009) Journal of cellular physiology 219, 45-56. PMID: 19086031

2. Glorioso, N. et al. (2007) Circulation research 100, 1522-9. PMID: 17446437

Note

For research use only.

图片

参考文献

产品说明书

Anti-EDN1/Endothelin1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-EDN1/Endothelin1 Polyclonal Antibody (HY182014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-EDN1/Endothelin1 Polyclonal Antibody (HY182014)

概述

图片

参考文献

产品说明书

相关产品推荐

获取报价