Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
Protein S100-A9 (S100A9) is a ~13 kDa protein. S100A9 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis, adhesion, can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2 and can induce degranulation of neutrophils by a MAPK-dependent mechanism. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Also participates in regulatory T-cell differentiation together with CD69.
1. Ryckman, C. et al. (2003) Journal of immunology (Baltimore, Md. : 1950) 170, 3233-42. PMID: 12626582
2. Vogl, T. et al. (2004) Blood 104, 4260-8. PMID: 15331440
3. Nakatani, Y. et al. (2005) Mediators of inflammation 2005, 280-92. PMID: 16258195
4. Champaiboon, C. et al. (2009) The Journal of biological chemistry 284, 7078-90. PMID: 19122197
5. Simard, JC. et al. (2010) Journal of leukocyte biology 87, 905-14. PMID: 20103766
6. Simard, JC. et al. (2011) Journal of immunology (Baltimore, Md. : 1950) 186, 3622-31. PMID: 21325622
7. Miyasaki, KT. et al. (1993) Journal of dental research 72, 517-23. PMID: 8423249
8. Lin, CR. et al. (2015) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 29, 5006-17. PMID: 26296369
Note
For research use only.
图片
Western blot
Western blot analysis was performed using anti-S100A9 polyclonal antibody at 1ug/mL on various samples. Lane 1: THP-1 cell lysate Lane 2: MCF-7 cell lysate
