Anti-GPX1 Polyclonal Antibody (HY365014)

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规格：

50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HY365014

品牌

abinScience

描述

Anti-GPX1 Polyclonal Antibody (HY365014) is a rabbit polyclonal antibody detecting GPX1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Callithrix jacchus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human GPX1 (Thr50-Ala202).

靶标

Cellular glutathione peroxidase, Glutathione peroxidase 1, GPx-1, GPX1, GSHPx-1

纯化方式

Purified by antigen affinity column.

Accession号

P07203

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Glutathione peroxidase 1 (GPX1) is a ~22 kDa protein. Catalyzes the reduction of hydroperoxides in a glutathione-dependent manner thus regulating cellular redox homeostasis. Can reduce small soluble hydroperoxides such as H2O2, cumene hydroperoxide and tert-butyl hydroperoxide, as well as several fatty acid-derived hydroperoxides. In platelets catalyzes the reduction of 12-hydroperoxyeicosatetraenoic acid, the primary product of the arachidonate 12-lipoxygenase pathway.

1. Sutherland, M. et al. (2001) The Biochemical journal 353, 91-100. PMID: 11115402

2. Schwarz, M. et al. (2023) Redox biology 59, 102593. PMID: 36608588

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-GPX1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: HL-60 cell lysate
Lane 2: Mouse liver lysate
Lane 3: Mouse kidney lysate
Lane 4: Mouse spleen lysate
Lane 5: Mouse lung lysate
Lane 6: Rat liver lysate
Lane 7: Rat kidney lysate

参考文献

产品说明书

Anti-GPX1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-GPX1 Polyclonal Antibody (HY365014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-GPX1 Polyclonal Antibody (HY365014)

概述

图片

参考文献

产品说明书

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