Anti-DLD Polyclonal Antibody (HY347014)

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概述
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产品说明书

概述

货号

HY347014

品牌

abinScience

描述

Anti-DLD Polyclonal Antibody (HY347014) is a rabbit polyclonal antibody detecting DLD in ELISA, IHC, WB. Suitable for Human, Mouse, Dog, Rat, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human DLD (Lys283-His487).

靶标

Glycine cleavage system L protein, Dihydrolipoamide dehydrogenase, GCSL, DLD, PHE3, Dihydrolipoyl dehydrogenase, mitochondrial, LAD

纯化方式

Purified by antigen affinity column.

Accession号

P09622

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Dihydrolipoyl dehydrogenase, mitochondrial (DLD) is a ~54 kDa protein. Lipoamide dehydrogenase is a component of the glycine cleavage system as well as an E3 component of three alpha-ketoacid dehydrogenase complexes (pyruvate-, alpha-ketoglutarate-, and branched-chain amino acid-dehydrogenase complex). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A. In monomeric form may have additional moonlighting function as serine protease. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.

1. Odièvre, MH. et al. (2005) Human mutation 25, 323-4. PMID: 15712224

2. Brautigam, CA. et al. (2006) Structure (London, England : 1993) 14, 611-21. PMID: 16442803

3. Cameron, JM. et al. (2006) American journal of medical genetics. Part A 140, 1542-52. PMID: 16770810

4. Babady, NE. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 6158-63. PMID: 17404228

5. Patel, MS. et al. (2009) Journal of molecular catalysis. B, Enzymatic 61, 2-6. PMID: 20160912

6. Park, YH. et al. (2010) Biochemical and biophysical research communications 395, 416-9. PMID: 20385101

7. Wang, Y. et al. (2017) Nature 552, 273-277. PMID: 29211711

Note

For research use only.

图片

参考文献

产品说明书

Anti-DLD Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-DLD Polyclonal Antibody (HY347014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-DLD Polyclonal Antibody (HY347014)

概述

图片

参考文献

产品说明书

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