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Anti-HSPD1 Polyclonal Antibody (HY166014)

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概述
货号HY166014
品牌abinScience
描述
Anti-HSPD1 Polyclonal Antibody (HY166014) is a rabbit polyclonal antibody detecting HSPD1 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Cricetulus griseus, and Chicken.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
种属反应性Human, Mouse, Rat
应用ELISA, IHC, WB
宿主Rabbit
同种型IgG
克隆类型Polyclonal
免疫原E. coli - derived recombinant Human HSPD1 (Ala27-Phe573).
靶标60 kDa heat shock protein, mitochondrial, HSP-60, HuCHA60, Chaperonin 60, HSP60, Mitochondrial matrix protein P1, Hsp60, HSPD1, 60 kDa chaperonin, CPN60, P60 lymphocyte protein, Heat shock protein 60
纯化方式Purified by antigen affinity column.
Accession号P10809
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

60 kDa heat shock protein, mitochondrial (HSPD1) is a ~61 kDa protein. Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10.

1. Levy-Rimler, G. et al. (2001) European journal of biochemistry 268, 3465-72. PMID: 11422376
2. Viitanen, PV. et al. (1992) The Journal of biological chemistry 267, 695-8. PMID: 1346131
NoteFor research use only.
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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