Anti-CKMT1A Polyclonal Antibody (HY409014)

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100ug

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概述
图片
参考文献
产品说明书

概述

货号

HY409014

品牌

abinScience

描述

Anti-CKMT1A Polyclonal Antibody (HY409014) is a rabbit polyclonal antibody detecting CKMT1A in ELISA, IHC, WB. Suitable for Bovine, Chicken, Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human CKMT1A (Ser41-Ile250).

靶标

U-MtCK, CKMT1A, CKMT, Acidic-type mitochondrial creatine kinase, Ubiquitous mitochondrial creatine kinase, Mia-CK, Creatine kinase U-type, mitochondrial

纯化方式

Purified by antigen affinity column.

Accession号

P12532

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Creatine kinase U-type, mitochondrial (CKMT1A) is a ~47 kDa protein. Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Note

For research use only.

图片

Immunofluorescence

Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting CKMT1A (HY409014) at a concentration of 12.4 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of CKMT1A was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

参考文献

Protein profile of Dabry’s Sturgeon (Acipenser dabryanus) spermatozoa and relationship to sperm quality

产品说明书

Anti-CKMT1A Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-CKMT1A Polyclonal Antibody (HY409014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-CKMT1A Polyclonal Antibody (HY409014)

概述

图片

参考文献

产品说明书

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