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Anti-ETFA Polyclonal Antibody (HB633014)

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概述
货号HB633014
品牌abinScience
描述
Anti-ETFA Polyclonal Antibody (HB633014) is a rabbit polyclonal antibody detecting ETFA in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Macaca fascicularis.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
种属反应性Human, Mouse, Rat
应用ELISA, IHC, WB
宿主Rabbit
同种型IgG
克隆类型Polyclonal
免疫原E. coli - derived recombinant Human ETFA (Ser52-Thr295).
靶标Alpha-ETF, ETFA, Electron transfer flavoprotein subunit alpha, mitochondrial
纯化方式Purified by antigen affinity column.
Accession号P13804
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Electron transfer flavoprotein subunit alpha, mitochondrial (ETFA) is a ~35 kDa protein. Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism.

1. Bross, P. et al. (1999) Molecular genetics and metabolism 67, 138-47. PMID: 10356313
2. Toogood, HS. et al. (2004) The Journal of biological chemistry 279, 32904-12. PMID: 15159392
3. Toogood, HS. et al. (2005) The Journal of biological chemistry 280, 30361-6. PMID: 15975918
4. Floyd, BJ. et al. (2016) Molecular cell 63, 621-632. PMID: 27499296
5. Salazar, D. et al. (1997) The Journal of biological chemistry 272, 26425-33. PMID: 9334218
6. Olsen, RK. et al. (2003) Human mutation 22, 12-23. PMID: 12815589
7. Freneaux, E. et al. (1992) The Journal of clinical investigation 90, 1679-86. PMID: 1430199
8. Indo, Y. et al. (1991) American journal of human genetics 49, 575-80. PMID: 1882842
NoteFor research use only.
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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