Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
Phospholipase A2, membrane associated (PLA2G2A) is a ~16 kDa protein. Secretory calcium-dependent phospholipase A2 that primarily targets extracellular phospholipids with implications in host antimicrobial defense, inflammatory response and tissue regeneration. Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) with preference for phosphatidylethanolamines and phosphatidylglycerols over phosphatidylcholines. Contributes to lipid remodeling of cellular membranes and generation of lipid mediators involved in pathogen clearance. Displays bactericidal activity against Gram-positive bacteria by directly hydrolyzing phospholipids of the bacterial membrane. Upon sterile inflammation, targets membrane phospholipids of extracellular mitochondria released from activated platelets, generating free unsaturated fatty acids such as arachidonate that is used by neighboring leukocytes to synthesize inflammatory eicosanoids such as leukotrienes.
1. Ishizaki, J. et al. (1999) The Journal of biological chemistry 274, 24973-9. PMID: 10455175
2. Suzuki, N. et al. (2000) The Journal of biological chemistry 275, 5785-93. PMID: 10681567
3. Kramer, RM. et al. (1989) The Journal of biological chemistry 264, 5768-75. PMID: 2925633
4. Laine, VJ. et al. (1999) Journal of immunology (Baltimore, Md. : 1950) 162, 7402-8. PMID: 10358193
5. Koduri, RS. et al. (2002) The Journal of biological chemistry 277, 5849-57. PMID: 11694541
