Anti-BRAF Polyclonal Antibody (HB617014)

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50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HB617014

品牌

abinScience

描述

Anti-BRAF Polyclonal Antibody (HB617014) is a rabbit polyclonal antibody detecting BRAF in ELISA, IHC, WB. Suitable for Coturnix japonica, Chicken, Human, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human BRAF (Asn500-His766).

靶标

BRAF1, RAFB1, Proto-oncogene B-Raf, BRAF, v-Raf murine sarcoma viral oncogene homolog B1, p94, Serine/threonine-protein kinase B-raf

纯化方式

Purified by antigen affinity column.

Accession号

P15056

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Serine/threonine-protein kinase B-raf (BRAF) is a ~84 kDa protein. Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway. Phosphorylates PFKFB2. May play a role in the postsynaptic responses of hippocampal neurons.

1. Brennan, DF. et al. (2011) Nature 472, 366-9. PMID: 21441910

2. Lavoie, H. et al. (2018) Nature 554, 549-553. PMID: 29433126

3. McGrail, K. et al. (2022) Nature communications 13, 7113. PMID: 36402789

4. Stephens, RM. et al. (1992) Molecular and cellular biology 12, 3733-42. PMID: 1508179

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-BRAF polyclonal antibody at 1ug/mL on various samples.
Lane 1: K562 cell lysate

参考文献

产品说明书

Anti-BRAF Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-BRAF Polyclonal Antibody (HB617014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-BRAF Polyclonal Antibody (HB617014)

概述

图片

参考文献

产品说明书

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