Anti-RAB5A Polyclonal Antibody (HB244014)

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100ug

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概述
图片
参考文献
产品说明书

概述

货号

HB244014

品牌

abinScience

描述

Anti-RAB5A Polyclonal Antibody (HB244014) is a rabbit polyclonal antibody detecting RAB5A in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Dog.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human RAB5A (Asp136-Asn215).

靶标

Ras-related protein Rab-5A, RAB5A, RAB5

纯化方式

Purified by antigen affinity column.

Accession号

P20339

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Ras-related protein Rab-5A (RAB5A) is a ~23 kDa protein. The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes and involved in early endocytic trafficking. Required for EEA1 recruitment to early endosomes. Recruits FERRY complex to early endosomes, where FERRY links early endosomes with a subgroup of mRNAs to enable mRNA intracellular distribution.

1. Hoffenberg, S. et al. (2000) The Journal of biological chemistry 275, 24661-9. PMID: 10818110

2. Fouraux, MA. et al. (2004) Molecular biology of the cell 15, 611-24. PMID: 14617813

3. Zhu, G. et al. (2004) Nature structural & molecular biology 11, 975-83. PMID: 15378032

4. Haas, AK. et al. (2005) Nature cell biology 7, 887-93. PMID: 16086013

5. Hunker, CM. et al. (2006) Biochemical and biophysical research communications 340, 967-75. PMID: 16410077

6. Fuchs, E. et al. (2007) The Journal of cell biology 177, 1133-43. PMID: 17562788

7. Quentin, D. et al. (2023) Molecular cell 83, 1856-1871.e9. PMID: 37267906

8. Gauthier-Campbell, C. et al. (2004) Molecular biology of the cell 15, 2205-17. PMID: 14978216

9. Baietti, MF. et al. (2012) Nature cell biology 14, 677-85. PMID: 22660413

Note

For research use only.

图片

参考文献

产品说明书

Anti-RAB5A Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-RAB5A Polyclonal Antibody (HB244014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-RAB5A Polyclonal Antibody (HB244014)

概述

图片

参考文献

产品说明书

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