Anti-Human Calreticulin Polyclonal Antibody (HB190014)

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规格：

50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HB190014

品牌

abinScience

描述

Anti-Calreticulin/CALR Polyclonal Antibody (HB190014) is a rabbit polyclonal antibody detecting Calreticulin in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Cricetulus griseus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human Calreticulin/CALR (Glu18-Pro204).

靶标

Calreticulin, ERp60, CRTC, Calregulin, grp60, HACBP, Endoplasmic reticulum resident protein 60, CALR, CRP55

内毒素水平

Please contact with the lab for this information.

纯度

>95% as determined by SDS-PAGE.

纯化方式

Purified by antigen affinity column.

Accession号

P27797

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Calreticulin is a ~48 kDa protein. Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis.

1. Nauseef, WM. et al. (1995) The Journal of biological chemistry 270, 4741-7. PMID: 7876246

2. Holaska, JM. et al. (2001) The Journal of cell biology 152, 127-40. PMID: 11149926

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-CALR polyclonal antibody at 1ug/mL on various samples.
Lane 1: SH-SY5Y cell lysate
Lane 2: Hela cell lysate
Lane 3: HL-60 cell lysate

参考文献

产品说明书

Anti-Human Calreticulin Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human Calreticulin Polyclonal Antibody (HB190014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human Calreticulin Polyclonal Antibody (HB190014)

概述

图片

参考文献

产品说明书

相关产品推荐

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