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Anti-CFP Polyclonal Antibody (HB792014)

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  • 参考文献

  • 产品说明书

概述
货号HB792014
品牌abinScience
描述
Anti-CFP Polyclonal Antibody (HB792014) is a rabbit polyclonal antibody detecting CFP in ELISA, IHC, WB. Suitable for Human and Pongo abelii.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
种属反应性Human
应用ELISA, IHC, WB
宿主Rabbit
同种型IgG
克隆类型Polyclonal
免疫原E. coli - derived recombinant Human CFP (Gln65-Cys190).
靶标PFC, CFP, Properdin, Complement factor P
纯化方式Purified by antigen affinity column.
Accession号P27918
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Properdin (CFP) is a ~51 kDa protein. A positive regulator of the alternate pathway (AP) of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes. Inhibits CFI-CFH mediated degradation of Complement C3 beta chain (C3b).

1. Hourcade, DE. (2006) The Journal of biological chemistry 281, 2128-32. PMID: 16301317
2. Ferreira, VP. et al. (2010) Immunobiology 215, 932-40. PMID: 20382442
3. Pedersen, DV. et al. (2017) The EMBO journal 36, 1084-1099. PMID: 28264884
4. Hourcade, DE. et al. (1998) The Journal of biological chemistry 273, 25996-6000. PMID: 9748277
5. Pedersen, DV. et al. (2019) Frontiers in immunology 10, 2007. PMID: 31507604
NoteFor research use only.
图片
  • Western blot

    Western blot analysis was performed using anti-CFP polyclonal antibody at 1μg/ml on various samples.
    Lane 1: Mouse brain lysate
    Lane 2: Rat brain lysate

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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