Anti-ERCC5 Polyclonal Antibody (HB668014)

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概述
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参考文献
产品说明书

概述

货号

HB668014

品牌

abinScience

描述

Anti-ERCC5 Polyclonal Antibody (HB668014) is a rabbit polyclonal antibody detecting ERCC5 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human ERCC5 (Leu778-Ala986).

靶标

ERCC5, DNA excision repair protein ERCC-5, ERCM2, XPGC, Xeroderma pigmentosum group G-complementing protein, XPG, DNA repair protein complementing XP-G cells

纯化方式

Purified by antigen affinity column.

Accession号

P28715

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

DNA excision repair protein ERCC-5 (ERCC5) is a ~133 kDa protein. Single-stranded structure-specific DNA endonuclease involved in DNA excision repair. Makes the 3'incision in DNA nucleotide excision repair (NER). Binds and bends DNA repair bubble substrate and breaks base stacking at the single-strand/double-strand DNA junction of the DNA bubble. Plays a role in base excision repair (BER) by promoting the binding of DNA glycosylase NTHL1 to its substrate and increasing NTHL1 catalytic activity that removes oxidized pyrimidines from DNA. Involved in transcription-coupled nucleotide excision repair (TCR) which allows RNA polymerase II-blocking lesions to be rapidly removed from the transcribed strand of active genes.

1. Tsutakawa, SE. et al. (2020) Proceedings of the National Academy of Sciences of the United States of America 117, 14127-14138. PMID: 32522879

2. González-Corrochano, R. et al. (2020) Nucleic acids research 48, 9943-9958. PMID: 32821917

3. Cloud, KG. et al. (1995) Mutation research 347, 55-60. PMID: 7651464

4. Habraken, Y. et al. (1994) Nucleic acids research 22, 3312-6. PMID: 8078765

5. O'Donovan, A. et al. (1994) Nature 371, 432-5. PMID: 8090225

6. O'Donovan, A. et al. (1994) The Journal of biological chemistry 269, 15965-8. PMID: 8206890

7. Bessho, T. (1999) Nucleic acids research 27, 979-83. PMID: 9927729

8. Sarker, AH. et al. (2005) Molecular cell 20, 187-98. PMID: 16246722

10. Kokic, G. et al. (2019) Nature communications 10, 2885. PMID: 31253769

Note

For research use only.

图片

参考文献

产品说明书

Anti-ERCC5 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-ERCC5 Polyclonal Antibody (HB668014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-ERCC5 Polyclonal Antibody (HB668014)

概述

图片

参考文献

产品说明书

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