Anti-PPIF Polyclonal Antibody (HB283014)

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100ug

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概述
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参考文献
产品说明书

概述

货号

HB283014

品牌

abinScience

描述

Anti-PPIF Polyclonal Antibody (HB283014) is a rabbit polyclonal antibody detecting PPIF in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human PPIF (Asn45-Lys196).

靶标

CypD, Peptidyl-prolyl cis-trans isomerase F, mitochondrial, Cyclophilin D, Cyclophilin F, CYP3, CyP-M, Rotamase F, PPIF, CyP-D, PPIase F, Mitochondrial cyclophilin

纯化方式

Purified by antigen affinity column.

Accession号

P30405

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Peptidyl-prolyl cis-trans isomerase F, mitochondrial (PPIF) is a ~22 kDa protein. PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding. Involved in regulation of the mitochondrial permeability transition pore (mPTP). It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis; the requirement of the PPIase activity for this function is debated. In cooperation with mitochondrial p53/TP53 is involved in activating oxidative stress-induced necrosis. Involved in modulation of mitochondrial membrane F(1)F(0) ATP synthase activity and regulation of mitochondrial matrix adenine nucleotide levels.

1. Davis, TL. et al. (2010) PLoS biology 8, e1000439. PMID: 20676357

2. Shanmughapriya, S. et al. (2015) Molecular cell 60, 47-62. PMID: 26387735

3. Vaseva, AV. et al. (2012) Cell 149, 1536-48. PMID: 22726440

4. Eliseev, RA. et al. (2009) The Journal of biological chemistry 284, 9692-9. PMID: 19228691

Note

For research use only.

图片

参考文献

产品说明书

Anti-PPIF Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-PPIF Polyclonal Antibody (HB283014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-PPIF Polyclonal Antibody (HB283014)

概述

图片

参考文献

产品说明书

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