Anti-AKT2 Polyclonal Antibody (HB933014)

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概述
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概述

货号

HB933014

品牌

abinScience

描述

Anti-AKT2 Polyclonal Antibody (HB933014) is a rabbit polyclonal antibody detecting AKT2 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human AKT2 (Met1-Lys146).

靶标

PKB beta, Protein kinase Akt-2, AKT2, Protein kinase B beta, RAC-beta serine/threonine-protein kinase, RAC-PK-beta

纯化方式

Purified by antigen affinity column.

Accession号

P31751

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

RAC-beta serine/threonine-protein kinase (AKT2) is a ~55 kDa protein. Serine/threonine kinase closely related to AKT1 and AKT3. All 3 enzymes, AKT1, AKT2 and AKT3, are collectively known as AKT kinase. AKT regulates many processes including metabolism, proliferation, cell survival, growth and angiogenesis, through the phosphorylation of a range of downstream substrates. Over 100 substrates have been reported so far, although for most of them, the precise AKT kinase catalyzing the reaction was not specified. AKT regulates glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface.

1. Heron-Milhavet, L. et al. (2011) Histology and histopathology 26, 651-62. PMID: 21432781

2. Hers, I. et al. (2011) Cellular signalling 23, 1515-27. PMID: 21620960

3. Walia, V. et al. (2019) Developmental cell 50, 229-246.e7. PMID: 31204173

4. Wolf, A. et al. (2013) Journal of cell science 126, 1832-44. PMID: 23444369

Note

For research use only.

图片

参考文献

产品说明书

Anti-AKT2 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-AKT2 Polyclonal Antibody (HB933014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-AKT2 Polyclonal Antibody (HB933014)

概述

图片

参考文献

产品说明书

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