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Anti-CASP14 Polyclonal Antibody (HB268014)

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概述
货号HB268014
品牌abinScience
描述
Anti-CASP14 Polyclonal Antibody (HB268014) is a rabbit polyclonal antibody detecting CASP14 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
种属反应性Human
应用ELISA, IHC, WB
宿主Rabbit
同种型IgG
克隆类型Polyclonal
免疫原E. coli - derived recombinant Human CASP14 (Met1-Gln242).
靶标CASP14, CASP-14, Caspase-14
纯化方式Purified by antigen affinity column.
Accession号P31944
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Caspase-14 (CASP14) is a ~27 kDa protein. Non-apoptotic caspase involved in epidermal differentiation. Is the predominant caspase in epidermal stratum corneum. Seems to play a role in keratinocyte differentiation and is required for cornification. Regulates maturation of the epidermis by proteolytically processing filaggrin. In vitro has a preference for the substrate [WY]-X-X-D motif and is active on the synthetic caspase substrate WEHD-ACF.

1. Fischer, H. et al. (2004) FEBS letters 577, 446-50. PMID: 15556625
2. Park, K. et al. (2006) Biochemical and biophysical research communications 347, 941-8. PMID: 16854378
3. Hibino, T. et al. (2010) Journal of cellular biochemistry 109, 487-97. PMID: 19960512
4. Beasley, S. et al. (2014) BioMed research international 2014, 417986. PMID: 25121097
5. Yamamoto-Tanaka, M. et al. (2014) Cell death & disease 5, e1181. PMID: 24743736
NoteFor research use only.
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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