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Anti-Human Flt-3/Flk-2 Polyclonal Antibody (HW794014)

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规格:
  • 50ug
  • 100ug
数量:
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  • 参考文献

  • 产品说明书

概述
货号HW794014
品牌abinScience
描述
Anti-CD135/FLT3 Polyclonal Antibody (HW794014) is a rabbit polyclonal antibody detecting CD135 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
种属反应性Human
应用ELISA, IHC, WB
宿主Rabbit
同种型IgG
克隆类型Polyclonal
免疫原E. coli - derived recombinant Human CD135/FLT3 (Asn27-Tyr376).
经测试应用WB
靶标FLT3, Stem cell tyrosine kinase 1, Fetal liver kinase-2, STK-1, STK1, FLK2, CD135, Receptor-type tyrosine-protein kinase FLT3, Fms-like tyrosine kinase 3, FL cytokine receptor, FLK-2, FLT-3
纯化方式Purified by antigen affinity column.
Accession号P36888
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Receptor-type tyrosine-protein kinase FLT3 (CD135) is a ~112 kDa protein. Tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B.

NoteFor research use only.
图片
  • Western blot

    Western blot analysis was performed using anti-FLT3 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse brain lysate
    Lane 2: Rat brain lysate

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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