Anti-DDOST Polyclonal Antibody (HW572014)

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规格：

50ug
100ug

数量：

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概述
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参考文献
产品说明书

概述

货号

HW572014

品牌

abinScience

描述

Anti-DDOST Polyclonal Antibody (HW572014) is a rabbit polyclonal antibody detecting DDOST in ELISA, IHC, WB. Suitable for Human, Mouse, Dog, Rat, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human DDOST (Ser43-Pro427).

靶标

DDOST 48 kDa subunit, OST48, Oligosaccharyl transferase 48 kDa subunit, KIAA0115, DDOST, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit

纯化方式

Purified by antigen affinity column.

Accession号

P39656

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit (DDOST) is a ~50 kDa protein. Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. Required for the assembly of both SST3A- and SS3B-containing OST complexes.

1. Ramírez, AS. et al. (2019) Science (New York, N.Y.) 366, 1372-1375. PMID: 31831667

2. Roboti, P. et al. (2012) Journal of cell science 125, 3474-84. PMID: 22467853

Note

For research use only.

图片

参考文献

产品说明书

Anti-DDOST Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-DDOST Polyclonal Antibody (HW572014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-DDOST Polyclonal Antibody (HW572014)

概述

图片

参考文献

产品说明书

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