Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
Reduced folate transporter (SLC19A1) is a ~64 kDa protein. Antiporter that mediates the import of reduced folates or a subset of cyclic dinucleotides, driven by the export of organic anions. Acts as an importer of immunoreactive cyclic dinucleotides, such as cyclic GMP-AMP (2'-3'-cGAMP), an immune messenger produced in response to DNA virus in the cytosol, and its linkage isomer 3'-3'-cGAMP, thus playing a role in triggering larger immune responses. Mechanistically, acts as a secondary active transporter, which exports intracellular organic anions down their concentration gradients to facilitate the uptake of its substrates. Has high affinity for N5-methyltetrahydrofolate, the predominant circulating form of folate. Also mediates the import of antifolate drug methotrexate.
1. Chancy, CD. et al. (2000) The Journal of biological chemistry 275, 20676-84. PMID: 10787414
2. Witt, TL. et al. (2004) The Journal of biological chemistry 279, 46755-63. PMID: 15337749
3. Hou, Z. et al. (2005) The Journal of biological chemistry 280, 36206-13. PMID: 16115875
4. Visentin, M. et al. (2012) Molecular pharmacology 82, 209-16. PMID: 22554803
5. Ritchie, C. et al. (2019) Molecular cell 75, 372-381.e5. PMID: 31126740
