Anti-ACAA2 Polyclonal Antibody (HW427014)

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概述
图片
参考文献
产品说明书

概述

货号

HW427014

品牌

abinScience

描述

Anti-ACAA2 Polyclonal Antibody (HW427014) is a rabbit polyclonal antibody detecting ACAA2 in ELISA, IHC, WB. Suitable for Bovine, Human, Mouse, Pongo abelii, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human ACAA2 (Arg90-Ala397).

靶标

Acetyl-CoA acyltransferase, Mitochondrial 3-oxoacyl-CoA thiolase, T1, Beta-ketothiolase, 3-ketoacyl-CoA thiolase, mitochondrial, Acyl-CoA hydrolase, mitochondrial, ACAA2, Acetyl-CoA acetyltransferase

纯化方式

Purified by antigen affinity column.

Accession号

P42765

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

3-ketoacyl-CoA thiolase, mitochondrial (ACAA2) is a ~41 kDa protein. In the production of energy from fats, this is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA (Probable). Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain unbranched 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms (Probable). Also catalyzes the condensation of two acetyl-CoA molecules into acetoacetyl-CoA and could be involved in the production of ketone bodies (Probable). Also displays hydrolase activity on various fatty acyl-CoAs. Thereby, could be responsible for the production of acetate in a side reaction to beta-oxidation (Probable).

1. Kiema, TR. et al. (2014) Acta crystallographica. Section D, Biological crystallography 70, 3212-25. PMID: 25478839

2. Cao, W. et al. (2008) Biochimica et biophysica acta 1780, 873-80. PMID: 18371312

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-ACAA2 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Mouse heart lysate
Lane 2: Mouse liver lysate
Lane 3: Rat heart lysate
Lane 4: Rat liver lysate

参考文献

产品说明书

Anti-ACAA2 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-ACAA2 Polyclonal Antibody (HW427014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-ACAA2 Polyclonal Antibody (HW427014)

概述

图片

参考文献

产品说明书

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