Anti-MAPK9 Polyclonal Antibody (HW530014) is a rabbit polyclonal antibody detecting MAPK9 in ELISA, IHC, WB. Suitable for Chicken, Human, Mouse, Rat, and Xenopus laevis.
Highlights
●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.
种属反应性
Human, Mouse, Rat
应用
ELISA, IHC, WB
宿主
Rabbit
同种型
IgG
克隆类型
Polyclonal
免疫原
E. coli - derived recombinant Human MAPK9 (Tyr26-Ile321).
靶标
Mitogen-activated protein kinase 9, MAPK 9, Stress-activated protein kinase 1a, SAPK1A, MAP kinase 9, SAPK1a, Stress-activated protein kinase JNK2, PRKM9, JNK-55, MAPK9, JNK2, c-Jun N-terminal kinase 2
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
Mitogen-activated protein kinase 9 (MAPK9) is a ~48 kDa protein. Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2. In turn, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. In response to oxidative or ribotoxic stresses, inhibits rRNA synthesis by phosphorylating and inactivating the RNA polymerase 1-specific transcription initiation factor RRN3.
1. De Graeve, F. et al. (1999) Oncogene 18, 3491-500. PMID: 10376527
2. Mayer, C. et al. (2005) Genes & development 19, 933-41. PMID: 15805466
