Anti-DGKE Polyclonal Antibody (HW773014)

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概述
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参考文献
产品说明书

概述

货号

HW773014

品牌

abinScience

描述

Anti-DGKE Polyclonal Antibody (HW773014) is a rabbit polyclonal antibody detecting DGKE in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human DGKE (Leu243-Gly502).

靶标

DGKE, Diglyceride kinase epsilon, DAG kinase epsilon, DGK-epsilon, Diacylglycerol kinase epsilon, DAGK5

纯化方式

Purified by antigen affinity column.

Accession号

P52429

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Diacylglycerol kinase epsilon (DGKE) is a ~63 kDa protein. Membrane-bound diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Also plays an important role in the biosynthesis of complex lipids. Displays specificity for diacylglycerol substrates with an arachidonoyl acyl chain at the sn-2 position, with the highest activity toward 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol the main diacylglycerol intermediate within the phosphatidylinositol turnover cycle. Can also phosphorylate diacylglycerol substrates with a linoleoyl acyl chain at the sn-2 position but much less efficiently.

1. Epand, RM. et al. (2004) Biochemistry 43, 14778-83. PMID: 15544348

2. Lung, M. et al. (2009) The Journal of biological chemistry 284, 31062-73. PMID: 19744926

3. Shulga, YV. et al. (2011) Journal of molecular biology 409, 101-12. PMID: 21477596

4. Shulga, YV. et al. (2011) FEBS letters 585, 4025-8. PMID: 22108654

5. Sato, M. et al. (2013) Pharmacology 92, 99-107. PMID: 23949095

6. Tang, W. et al. (1996) The Journal of biological chemistry 271, 10237-41. PMID: 8626589

Note

For research use only.

图片

参考文献

产品说明书

Anti-DGKE Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-DGKE Polyclonal Antibody (HW773014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-DGKE Polyclonal Antibody (HW773014)

概述

图片

参考文献

产品说明书

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