Anti-EEF1A1 Polyclonal Antibody (HX255014)

价格:

规格：

50ug
100ug

数量：

联系我们

一键复制产品信息

概述
图片
参考文献
产品说明书

概述

货号

HX255014

品牌

abinScience

描述

Anti-EEF1A1 Polyclonal Antibody (HX255014) is a rabbit polyclonal antibody detecting EEF1A1 in ELISA, IHC, WB. Suitable for Human, Mouse, Cat, Rat, and Adisura bella.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human EEF1A1 (Met1-Pro241).

靶标

EEF1A, Elongation factor Tu, Leukocyte receptor cluster member 7, Elongation factor 1-alpha 1, EF-1-alpha-1, Eukaryotic elongation factor 1 A-1, EF-Tu, EF1A, eEF1A-1, LENG7, EEF1A1

纯化方式

Purified by antigen affinity column.

Accession号

P68104

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Elongation factor 1-alpha 1 (EEF1A1) is a ~50 kDa protein. Translation elongation factor that catalyzes the GTP-dependent binding of aminoacyl-tRNA (aa-tRNA) to the A-site of ribosomes during the elongation phase of protein synthesis. Base pairing between the mRNA codon and the aa-tRNA anticodon promotes GTP hydrolysis, releasing the aa-tRNA from EEF1A1 and allowing its accommodation into the ribosome. The growing protein chain is subsequently transferred from the P-site peptidyl tRNA to the A-site aa-tRNA, extending it by one amino acid through ribosome-catalyzed peptide bond formation. Also plays a role in the positive regulation of IFNG transcription in T-helper 1 cells as part of an IFNG promoter-binding complex with TXK and PARP1. Also plays a role in cytoskeleton organization by promoting actin bundling.

1. Ferguson, A. et al. (2015) Molecular cell 60, 475-86. PMID: 26593721

2. Carelli, JD. et al. (2015) eLife 4. PMID: 26651998

3. Wang, HY. et al. (2022) Nature chemistry 14, 1443-1450. PMID: 36123449

4. Juette, MF. et al. (2022) eLife 11. PMID: 36264623

5. Oltion, K. et al. (2023) Cell 186, 346-362.e17. PMID: 36638793

6. Maruyama, T. et al. (2007) Clinical and experimental immunology 147, 164-75. PMID: 17177976

Note

For research use only.

图片

参考文献

产品说明书

Anti-EEF1A1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-EEF1A1 Polyclonal Antibody (HX255014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-EEF1A1 Polyclonal Antibody (HX255014)

概述

图片

参考文献

产品说明书

相关产品推荐

获取报价