Anti-Human MCAM/CD146 Recombinant Antibody (SAA0060) (HW484207)

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货号	HW484207
品牌	abinScience
种属反应性	Human
应用	FCM
同种型	IgG1, kappa
克隆号	SAA0060
经测试应用	FCM
靶标	CD146, Melanoma cell adhesion molecule, S-endo 1 endothelial-associated antigen, Melanoma-associated antigen A32, MUC18, MCAM, Melanoma-associated antigen MUC18, Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12
内毒素水平	Please contact with the lab for this information.
纯度	>95% as determined by SDS-PAGE.
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	P43121
状态	Liquid
保存溶液	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景	Cell surface glycoprotein MUC18 (CD146/MCAM) is a ~71 kDa protein. Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as a surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
Note	For research use only.

Bioactivity

SEC-HPLC detection for Anti-Human CD146/MUC18/MCAM Antibody (ABS1541).
Flow Cytometry

Flow-cytometry using anti-human CD146 antibody. Human HeLa cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human CD146 antibody monoclonal antibody (Catalog # HW484207 , Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

产品说明书

Anti-Human MCAM/CD146 Recombinant Antibody (SAA0060).pdf

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Anti-Human MCAM/CD146 Recombinant Antibody (SAA0060) (HW484207)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

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实验相关试剂

Anti-Human MCAM/CD146 Recombinant Antibody (SAA0060) (HW484207)

概述

图片

参考文献

产品说明书

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