Anti-Human EMMPRIN/CD147 Recombinant Antibody (SAA0061) (HW664107)

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货号	HW664107
品牌	abinScience
种属反应性	Human
应用	ELISA, FCM, IHC, WB
同种型	IgG1, kappa
克隆号	SAA0061
经测试应用	WB, FCM
靶标	Hepatoma-associated antigen, BSG, HAb18G, 5F7, TCSF, OK blood group antigen, Collagenase stimulatory factor, CD147, Leukocyte activation antigen M6, Basigin, Extracellular matrix metalloproteinase inducer, EMMPRIN, Tumor cell-derived collagenase stimulatory factor
内毒素水平	Please contact with the lab for this information.
纯度	>95% as determined by SDS-PAGE.
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	P35613
状态	Liquid
保存溶液	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景	Basigin (CD147/BSG) is a ~42 kDa protein. Essential for normal retinal maturation and development. Acts as a retinal cell surface receptor for NXNL1 and plays an important role in NXNL1-mediated survival of retinal cone photoreceptors. In association with glucose transporter SLC16A1/GLUT1 and NXNL1, promotes retinal cone survival by enhancing aerobic glycolysis and accelerating the entry of glucose into photoreceptors. May act as a potent stimulator of IL6 secretion in multiple cell lines that include monocytes. 1. Aït-Ali, N. et al. (2015) Cell 161, 817-32. PMID: 25957687 2. Redzic, JS. et al. (2011) Journal of molecular biology 411, 68-82. PMID: 21620857
Note	For research use only.

Bioactivity

SEC-HPLC detection for Anti-Human CD147/BSG Antibody (ABS1891).
Flow Cytometry

Flow-cytometry using anti-human CD147 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD147 monoclonal antibody (Catalog HW664107, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CD147/BSG monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human CD147/BSG (Catalog No: HW664011)

产品说明书

Anti-Human EMMPRIN/CD147 Recombinant Antibody (SAA0061).pdf

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Anti-Human EMMPRIN/CD147 Recombinant Antibody (SAA0061) (HW664107)

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参考文献

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重组蛋白

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实验相关试剂

Anti-Human EMMPRIN/CD147 Recombinant Antibody (SAA0061) (HW664107)

概述

图片

参考文献

产品说明书

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