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Anti-Human CXCR5 Recombinant Antibody (16D7) (HB160107)

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概述
货号HB160107
品牌abinScience
种属反应性Human
应用FCM
同种型IgG1, kappa
克隆号16D7
靶标CXC-R5, CD185, MDR-15, C-X-C chemokine receptor type 5, CXCR5, CXCR-5, MDR15, Monocyte-derived receptor 15, Burkitt lymphoma receptor 1, BLR1
内毒素水平Please contact with the lab for this information.
纯度>95% as determined by SDS-PAGE.
纯化方式Protein A/G purified from cell culture supernatant.
Accession号P32302
状态Liquid
保存溶液 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

稳定性和存储Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景

C-X-C chemokine receptor type 5 (CD185/CXCR5) is a ~41 kDa protein. Cytokine receptor that binds to B-lymphocyte chemoattractant (BLC). Involved in B-cell migration into B-cell follicles of spleen and Peyer patches but not into those of mesenteric or peripheral lymph nodes. May have a regulatory function in Burkitt lymphoma (BL) lymphomagenesis and/or B-cell differentiation.

NoteFor research use only.
图片
  • Bioactivity

    SEC-HPLC detection for Anti-Human CD185/CXCR5 Antibody (16D7).

  • Flow Cytometry

    Flow-cytometry using anti-Human CD185/CXCR5 antibody. 293T cells transfected with CD185/CXCR5 (Red Histogram) or Empty vector (Yellow Histogram) with an anti-human CD185/CXCR5 antibody monoclonal antibody (Catalog #HB160107) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF647 conjugated Goat anti-Mouse antibody and cells analysed on a NovoCyte Flow Cytometer.

  • Flow CytoMetry

    Flow-cytometry using anti-human CD185 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD185 monoclonal antibody (Catalog: HB160107) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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