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Anti-Human CD1a Recombinant Antibody (2113) (HY043107)

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概述
货号HY043107
品牌abinScience
种属反应性Human
应用FCM
同种型IgG1, kappa
克隆号2113
靶标T-cell surface glycoprotein CD1a, CD1A, T-cell surface antigen T6/Leu-6, CD1a, hTa1 thymocyte antigen
内毒素水平Please contact with the lab for this information.
纯度>95% as determined by SDS-PAGE.
纯化方式Protein A/G purified from cell culture supernatant.
Accession号P06126
状态Liquid
保存溶液 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

稳定性和存储Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景

T-cell surface glycoprotein CD1a is a ~37 kDa protein. Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.

文献1. Sousa SR, et al. Discovery and mode of action of a novel analgesic β-toxin from the African spider Ceratogyrus darlingi. PLoS One. 2017 Sep 7;12(9):e0182848. [HY043107]
NoteFor research use only.
图片
  • Bioactivity

    SEC-HPLC detection for Anti-Human CD1a Antibody (2113).

  • Flow Cytometry

    Flow-cytometry using anti-human CD1a antibody. Human MOLT-4 cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human CD1a antibody monoclonal antibody (Catalog # HY043107 , Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a CoraLite488 conjugated goat anti-mouse antibody (Catalog # MF690314) and cells analysed on a NovoCyte Flow Cytometer.

  • Flow Cytometry

    Flow-cytometry using anti-human CD1a antibody. Human Jurkat cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human CD1a antibody monoclonal antibody (Catalog # HY043107 , Red Histogram) at a concentration of 20µg/ml for 30 mins at RT. After washing, bound antibody was detected using a CoraLite488 conjugated goat anti-mouse antibody (Catalog # MF690314) and cells analysed on a NovoCyte Flow Cytometer.

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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