Anti-Human CD20 Recombinant Antibody (SAA0007) (HY257207)

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货号	HY257207
品牌	abinScience
种属反应性	Human
应用	FCM
同种型	IgG2b, kappa
克隆号	SAA0007
经测试应用	FCM
靶标	B-lymphocyte surface antigen B1, Membrane-spanning 4-domains subfamily A member 1, MS4A1, Leukocyte surface antigen Leu-16, B-lymphocyte antigen CD20, Bp35, CD20
内毒素水平	Please contact with the lab for this information.
纯度	>95% as determined by SDS-PAGE.
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	P11836
状态	Liquid
保存溶液	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景	B-lymphocyte antigen CD20 (MS4A1) is a ~33 kDa protein. B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR. CD20 is the therapeutic target of rituximab (MabThera) and obinutuzumab (Gazyva). 1. Li, H. et al. (2003) The Journal of biological chemistry 278, 42427-34. PMID: 12920111 2. Tedder, TF. et al. (1985) Journal of immunology (Baltimore, Md. : 1950) 135, 973-9. PMID: 3925015 3. Bubien, JK. et al. (1993) The Journal of cell biology 121, 1121-32. PMID: 7684739 4. Polyak, MJ. et al. (2008) The Journal of biological chemistry 283, 18545-52. PMID: 18474602
Note	For research use only.

Flow Cytometry

Flow-cytometry using anti-human CD20 antibody.CD20 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD20 antibody monoclonal antibody (Catalog # HY257207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CD20 antibody.Daudi cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD20 antibody monoclonal antibody (Catalog # HY257207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

产品说明书

Anti-Human CD20 Recombinant Antibody (SAA0007).pdf

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Anti-Human CD20 Recombinant Antibody (SAA0007) (HY257207)

概述

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参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human CD20 Recombinant Antibody (SAA0007) (HY257207)

概述

图片

参考文献

产品说明书

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