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Anti-Human CD39/ENTPD1 Recombinant Antibody (SRF367) (HW564107)

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概述
货号HW564107
品牌abinScience
种属反应性Human
应用FCM
同种型IgG1, kappa
克隆号SRF367
经测试应用FCM
靶标Ecto-ATP diphosphohydrolase 1, NTPDase 1, ENTPD1, Ecto-ATPase 1, Ecto-apyrase, Ecto-ATPDase 1, Ectonucleoside triphosphate diphosphohydrolase 1, CD39, Lymphoid cell activation antigen
内毒素水平Please contact with the lab for this information.
纯度>95% as determined by SDS-PAGE.
纯化方式Protein A/G purified from cell culture supernatant.
Accession号P49961
状态Liquid
保存溶液 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

稳定性和存储Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景

Ectonucleoside triphosphate diphosphohydrolase 1 (CD39/ENTPD1) is a ~57 kDa protein. Catalyzes the hydrolysis of nucleoside triphosphates (NTPs) and diphosphates (NDPs) (Probable). The enzyme sequentially removes phosphate groups in two successive steps, converting NTPs to nucleoside monophosphates (NMPs) via NDP intermediates (Probable). This activity contributes to the regulation of extracellular levels of nucleotides (Probable). By hydrolyzing proinflammatory ATP and platelet-activating ADP to AMP, it blocks platelet aggregation and supports blood flow.

1. Christoforidis, S. et al. (1995) European journal of biochemistry 234, 66-74. PMID: 8529670
2. Wang, TF. et al. (1996) The Journal of biological chemistry 271, 9898-901. PMID: 8626624
3. Kaczmarek, E. et al. (1996) The Journal of biological chemistry 271, 33116-22. PMID: 8955160
4. Robson, SC. et al. (1997) The Journal of experimental medicine 185, 153-63. PMID: 8996251
NoteFor research use only.
图片
  • Bioactivity

    SEC-HPLC detection for Anti-Human CD39/ENTPD1 Antibody (SRF367).

  • Flow Cytometry

    Flow-cytometry using anti-human CD39 antibody.CD39 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD39 antibody monoclonal antibody (Catalog # HW564107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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