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Anti-Human CEACAM6/CD66c Recombinant Antibody (SAA0029) (HW494107)

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概述
货号HW494107
品牌abinScience
种属反应性Human
应用FCM
同种型IgG1, kappa
克隆号SAA0029
经测试应用FCM
靶标Normal cross-reacting antigen, Carcinoembryonic antigen-related cell adhesion molecule 6, CEACAM6, CD66c, NCA, Non-specific crossreacting antigen
内毒素水平Please contact with the lab for this information.
纯度>95% as determined by SDS-PAGE.
纯化方式Protein A/G purified from cell culture supernatant.
Accession号P40199
状态Liquid
保存溶液 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

稳定性和存储Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景

Cell adhesion molecule CEACAM6 (CD66c) is a ~37 kDa protein. Cell surface glycoprotein that plays a role in cell adhesion and tumor progression. Intercellular adhesion occurs in a calcium- and fibronectin-independent manner. Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM5 and CEACAM8. Heterophilic interaction with CEACAM8 occurs in activated neutrophils. Plays a role in neutrophil adhesion to cytokine-activated endothelial cells.

1. Ordoñez, C. et al. (2000) Cancer research 60, 3419-24. PMID: 10910050
2. Kuroki, M. et al. (2001) Journal of leukocyte biology 70, 543-50. PMID: 11590190
3. Kuijpers, TW. et al. (1992) The Journal of cell biology 118, 457-66. PMID: 1378450
4. Blumenthal, RD. et al. (2005) Cancer research 65, 8809-17. PMID: 16204051
5. Oikawa, S. et al. (1991) The Journal of biological chemistry 266, 7995-8001. PMID: 2022629
6. Oikawa, S. et al. (1989) Biochemical and biophysical research communications 164, 39-45. PMID: 2803308
7. Yamanaka, T. et al. (1996) Protein expression and purification 7, 438-46. PMID: 8776764
NoteFor research use only.
图片
  • Bioactivity

    SEC-HPLC detection for Anti-Human CD66c/CEACAM6 Antibody (ABS0697).

  • Flow CytoMetry

    Flow-cytometry using anti-human CD66c antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD66c monoclonal antibody (Catalog: HW494107) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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