Anti-Human 5'-Nucleotidase/CD73 Recombinant Antibody (SAA0032) (HB209107)

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货号	HB209107
品牌	abinScience
种属反应性	Human
应用	FCM
同种型	IgG1, lambda
克隆号	SAA0032
经测试应用	FCM
靶标	CD73, 5'-nucleotidase, NT5E, NT5, 5'-NT, Ecto-5'-nucleotidase, NTE
内毒素水平	Please contact with the lab for this information.
纯度	>95% as determined by SDS-PAGE.
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	P21589
状态	Liquid
保存溶液	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景	5'-nucleotidase (CD73/NT5E) is a ~63 kDa protein. Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate. Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms. Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN. 1. Garavaglia, S. et al. (2012) The Biochemical journal 441, 131-41. PMID: 21933152 2. Heuts, DP. et al. (2012) Chembiochem : a European journal of chemical biology 13, 2384-91. PMID: 22997138 3. Knapp, K. et al. (2012) Structure (London, England : 1993) 20, 2161-73. PMID: 23142347 4. Fausther, M. et al. (2014) PloS one 9, e98568. PMID: 24887587 5. Scaletti, E. et al. (2021) Purinergic signalling 17, 693-704. PMID: 34403084
Note	For research use only.

Flow Cytometry

Flow-cytometry using anti-human CD73 antibody.U87 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD73 antibody monoclonal antibody (Catalog # HB209107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Bioactivity

Detects Recombinant Human CD73/NT5E Protein (Cat: HB209011) in indirect ELISA.

产品说明书

Anti-Human 5'-Nucleotidase/CD73 Recombinant Antibody (SAA0032).pdf

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Anti-Human 5'-Nucleotidase/CD73 Recombinant Antibody (SAA0032) (HB209107)

概述

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参考文献

产品说明书

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重组蛋白

抗体

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实验相关试剂

Anti-Human 5'-Nucleotidase/CD73 Recombinant Antibody (SAA0032) (HB209107)

概述

图片

参考文献

产品说明书

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