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Anti-Mouse CD16/CD32 Recombinant Antibody (2.4G2) (MP369107)

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概述
货号MP369107
品牌abinScience
种属反应性Mouse
应用FCM, FuncS, IF, IP
同种型IgG2b, kappa
克隆号2.4G2
靶标Low affinity immunoglobulin gamma Fc region receptor III, IgG Fc receptor III, Fc-gamma RIII, FcRIII, CD16, Low affinity immunoglobulin gamma Fc region receptor II, Fc gamma receptor IIB, Fc-gamma RII, Fc-gamma-RIIB, FcRII, IgG Fc receptor II beta, Lymphocyte antigen 17, Ly-17, CD32, Fcgr2, Fcgr2b, Ly-17
内毒素水平<1 EU/mg
纯度>95% as determined by SDS-PAGE.
相关产品 pH 7.4 Dilution Buffer
纯化方式Protein A/G purified from cell culture supernatant.
Accession号P08508, P08101
状态Liquid
保存溶液 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

重悬Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景

Low affinity immunoglobulin gamma Fc region receptor III (CD16/CD32) is a ~30 kDa protein. Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor which binds to IgG1, IgG2a and IgG2b. Mediates neutrophil activation by IgG complexes redundantly with Fcgr4.

1. Hirano, M. et al. (2007) Nature immunology 8, 762-71. PMID: 17558411
2. Jakus, Z. et al. (2008) Journal of immunology (Baltimore, Md. : 1950) 180, 618-29. PMID: 18097064
NoteFor research use only.
图片
  • Bioactivity

    Detects CD16/CD32 in indirect ELISAs.

  • Flow Cytometry

    Flow-cytometry using anti-mouse CD16 antibody.CD16 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-mouse CD16 antibody monoclonal antibody (Catalog # MP369107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-rat antibody and cells analysed on a NovoCyte Flow Cytometer.

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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