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Anti-Mouse CD40 Ligand/TNFSF5 Recombinant Antibody (MR1/MR-1) (MB199107)

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概述
货号MB199107
品牌abinScience
种属反应性Mouse
应用Blocking, FCM, IHC
同种型IgG1, kappa
克隆号MR1/MR-1
经测试应用FCM
靶标CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, TRAP, Tumor necrosis factor ligand superfamily member 5, CD154, Cd40lg, Cd40l, Tnfsf5
内毒素水平Please contact with the lab for this information.
纯度>95% as determined by SDS-PAGE.
纯化方式Protein A/G purified from cell culture supernatant.
Accession号P27548
状态Liquid
保存溶液 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

稳定性和存储Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景

CD40 ligand (CD154/CD40L) is a ~29 kDa protein. Cytokine that acts as a ligand to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B. Induces the activation of kinases MAPK8 and PAK2 in T-cells.

1. Armitage, RJ. et al. (1992) Nature 357, 80-2. PMID: 1374165
NoteFor research use only.
图片
  • Flow Cytometry

    Flow-cytometry using anti-mouse CD154 antibody.CD154 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-mouse CD154 antibody monoclonal antibody (Catalog # MB199107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-rat antibody and cells analysed on a NovoCyte Flow Cytometer.

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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