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Anti-Human Semaphorin 4D/CD100 Recombinant Antibody (SAA0331) (HV563207)

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规格:
  • 50ug
  • 100ug
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  • 产品说明书

概述
货号HV563207
品牌abinScience
种属反应性Human
应用FCM, FuncS, Neutralization
同种型IgG1, kappa
克隆号SAA0331
经测试应用FCM
靶标A8, Semaphorin-4D, GR3, BB18, SEMAJ, C9orf164, CD100, SEMA4D
内毒素水平Please contact with the lab for this information.
纯度>95% as determined by SDS-PAGE.
纯化方式Protein A/G purified from cell culture supernatant.
Accession号Q92854
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 0.09% Sodium azide.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

稳定性和存储Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景

Semaphorin-4D (CD100/SEMA4D) is a ~96 kDa protein. Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling. Regulates GABAergic synapse development. Promotes the development of inhibitory synapses in a PLXNB1-dependent manner. Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA. Promotes the migration of cerebellar granule cells.

1. Janssen, BJ. et al. (2010) Nature 467, 1118-22. PMID: 20877282
2. Vodrazka, P. et al. (2009) The European journal of neuroscience 30, 1193-208. PMID: 19788569
3. Basile, JR. et al. (2005) Molecular and cellular biology 25, 6889-98. PMID: 16055703
4. Hall, KT. et al. (1996) Proceedings of the National Academy of Sciences of the United States of America 93, 11780-5. PMID: 8876214
NoteFor research use only.
图片
  • Flow Cytometry

    Flow-cytometry using anti-human CD100 antibody.CD100 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD100 antibody monoclonal antibody (Catalog # HV563207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Flow Cytometry

    Flow-cytometry using anti-human CD100 antibody.Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD100 antibody monoclonal antibody (Catalog # HV563207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Flow Cytometry

    Flow-cytometry using anti-human CD100 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD100 antibody monoclonal antibody (Catalog # HV563207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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