Anti-Human IL-33 Recombinant Antibody (SAA0395) (HF543107)

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100ug

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货号	HF543107
品牌	abinScience
种属反应性	Human
应用	ELISA, FCM
同种型	IgG1, lambda
克隆号	SAA0395
经测试应用	FCM
靶标	NF-HEV, IL-1F11, C9orf26, IL-33, Interleukin-1 family member 11, IL33, Nuclear factor from high endothelial venules, NFHEV, Interleukin-33, IL1F11
内毒素水平	Please contact with the lab for this information.
纯度	>95% as determined by SDS-PAGE.
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	O95760
状态	Liquid
保存溶液	0.01M PBS, pH 7.4, 0.09% Sodium azide. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景	Interleukin-33 (IL33) is a ~30 kDa protein. Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as an enhancer of polarization of alternatively activated macrophages. Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury. 1. Schmitz, J. et al. (2005) Immunity 23, 479-90. PMID: 16286016 2. Kurowska-Stolarska, M. et al. (2009) Journal of immunology (Baltimore, Md. : 1950) 183, 6469-77. PMID: 19841166 3. Komai-Koma, M. et al. (2007) European journal of immunology 37, 2779-86. PMID: 17853410 4. Moussion, C. et al. (2008) PloS one 3, e3331. PMID: 18836528
Note	For research use only.

Flow-cytometry

Flow-cytometry using anti-human IL33 antibody.Human Jurkat cell line were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL33 antibody monoclonal antibody (Catalog # HF543107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry

Flow-cytometry using anti-human IL33 antibody.HUVEC cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL33 antibody monoclonal antibody (Catalog # HF543107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry

Flow-cytometry using anti-human IL33 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL33 antibody monoclonal antibody (Catalog # HF543107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

产品说明书

Anti-Human IL-33 Recombinant Antibody (SAA0395).pdf

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Anti-Human IL-33 Recombinant Antibody (SAA0395) (HF543107)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human IL-33 Recombinant Antibody (SAA0395) (HF543107)

概述

图片

参考文献

产品说明书

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