Anti-Human Nectin-4 Recombinant Antibody (SAA0403) (HV985207)

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100ug

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货号	HV985207
品牌	abinScience
种属反应性	Human
应用	ELISA, FCM, WB
同种型	IgG2a, kappa
克隆号	SAA0403
经测试应用	WB, FCM
靶标	LNIR, NECTIN4, PRR4 receptor-related protein 4, Nectin cell adhesion molecule 4, Ig superfamily receptor LNIR, Nectin-4, PVRL4
内毒素水平	Please contact with the lab for this information.
纯度	>95% as determined by SDS-PAGE.
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	Q96NY8
状态	Liquid
保存溶液	0.01M PBS, pH 7.4, 0.09% Sodium azide. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景	Nectin-4 (NECTIN4/PVRL4) is a ~55 kDa protein. Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells. NECTIN4 is the therapeutic target of enfortumab vedotin (Padcev).
Note	For research use only.

Bioactivity

Detects NECTIN4/PVRL4 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human NECTIN4 antibody.NECTIN4 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human NECTIN4 antibody monoclonal antibody (Catalog # HV985207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using anti-human NECTIN4 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human NECTIN4 monoclonal antibody (Catalog: HV985207) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-NECTIN4 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human NECTIN4 (Catalog No: HV985011)
Lane 2: negative control

产品说明书

Anti-Human Nectin-4 Recombinant Antibody (SAA0403).pdf

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Anti-Human Nectin-4 Recombinant Antibody (SAA0403) (HV985207)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

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实验相关试剂

Anti-Human Nectin-4 Recombinant Antibody (SAA0403) (HV985207)

概述

图片

参考文献

产品说明书

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