| 货号 | HV456107 |
|---|---|
| 品牌 | abinScience |
| 种属反应性 | Human |
| 应用 | ELISA, FCM |
| 宿主 | Mouse |
| 同种型 | IgG2a, kappa |
| 克隆号 | SAA0405 |
| 克隆类型 | Monoclonal |
| 靶标 | Herpesvirus entry mediator B, Herpes virus entry mediator B, Nectin cell adhesion molecule 2, HVEB, PVRL2 receptor-related protein 2, CD112, PRR2, Nectin-2, HveB, NECTIN2 |
| 内毒素水平 | Please contact with the lab for this information. |
| 纯度 | >95% as determined by SDS-PAGE. |
| 纯化方式 | Protein A/G purified from cell culture supernatant. |
| Accession号 | Q92692 |
| 状态 | Liquid |
| 保存溶液 | 0.01M PBS, pH 7.4, 0.09% Sodium azide. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Note | For research use only. |
Detects CD112/Nectin-2/PVRL2 in indirect ELISAs.
Flow-cytometry using anti-human Nectin2 antibody.K562 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Nectin2 antibody monoclonal antibody (Catalog # HV456107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human Nectin2 antibody.KG-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Nectin2 antibody monoclonal antibody (Catalog # HV456107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human CD112 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD112 monoclonal antibody (Catalog: HV456107) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot analysis was performed using anti-CD112/Nectin-2/PVRL2 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD112/Nectin-2/PVRL2 (Catalog No: HV456011)
Lane 2: negative control
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