Anti-Human CCL7/MCP-3 Recombinant Antibody (SAA0539) (HX155107)

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100ug

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货号	HX155107
品牌	abinScience
种属反应性	Human
应用	ELISA, FCM, WB
同种型	IgG2a, kappa
克隆号	SAA0539
靶标	C-C motif chemokine 7, MCP-3, CCL7, Monocyte chemotactic protein 3, Monocyte chemoattractant protein 3, NC28, Small-inducible cytokine A7, SCYA6, SCYA7, MCP3
内毒素水平	Please contact with the lab for this information.
纯度	>95% as determined by SDS-PAGE.
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	P80098
状态	Liquid
保存溶液	0.01M PBS, pH 7.4, 0.09% Sodium azide. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景	C-C motif chemokine 7 (CCL7/MCP-3) is a ~11 kDa protein. Chemotactic factor that plays an important role in immune regulation. Attracts monocytes, eosinophils, basophils, and T-cells to sites of inflammation or infection. Upon binding to various chemokine receptors including CCR1, CCR2, CCR3, and CCR5, facilitates immune cell migration by guiding them to infected tissues. Interacts with CCR2 to facilitate the release of monocytes from the bone marrow into the bloodstream, maintaining monocyte homeostasis. In turn, monocytes recruited to inflamed or injured tissues can differentiate into macrophages or dendritic cells, which are essential for immune defense and tissue repair. 1. Bonini, JA. et al. (1997) DNA and cell biology 16, 1249-56. PMID: 9364936 2. Blanpain, C. et al. (1999) Blood 94, 1899-905. PMID: 10477718 3. Wei, H. et al. (2024) Cellular signalling 117, 111122. PMID: 38417634
Note	For research use only.

Flow Cytometry

Flow-cytometry using anti-human CCL7 antibody.LPS treated THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CCL7 antibody monoclonal antibody (Catalog # HX155107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CCL24/Eotaxin-2/MPIF-2 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CCL24/Eotaxin-2/MPIF-2 (Catalog No: HT048012)
Lane 2: negative control

产品说明书

Anti-Human CCL7/MCP-3 Recombinant Antibody (SAA0539).pdf

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Anti-Human CCL7/MCP-3 Recombinant Antibody (SAA0539) (HX155107)

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参考文献

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重组蛋白

抗体

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实验相关试剂

Anti-Human CCL7/MCP-3 Recombinant Antibody (SAA0539) (HX155107)

概述

图片

参考文献

产品说明书

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